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Very High Density of Chinese Hamster Ovary Cells in Perfusion by Alternating Tangential Flow or Tangential Flow Filtration in WAVE Bioreactor™—Part II: Applications for Antibody Production and Cryopreservation

A high cell density perfusion process of monoclonal antibody (MAb) producing Chinese hamster ovary (CHO) cells was developed in disposable WAVE Bioreactor™ using external hollow fiber (HF) filter as cell separation device. Tangential flow filtration (TFF) and alternating tangential flow (ATF) system...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Clincke, Marie-Françoise, Mölleryd, Carin, Samani, Puneeth K, Lindskog, Eva, Fäldt, Eric, Walsh, Kieron, Chotteau, Véronique
Format: Artigo
Sprache:Inglês
Veröffentlicht: Blackwell Publishing Ltd 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3752935/
https://ncbi.nlm.nih.gov/pubmed/23436783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/btpr.1703
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