An Escherichia coli strain for expression of the connexin45 carboxyl terminus attached to the 4th transmembrane domain

A major problem for structural characterization of membrane proteins, such as connexins, by nuclear magnetic resonance (NMR) occurs at the initial step of the process, the production of sufficient amounts of protein. This occurs because proteins must be expressed in minimal based media. Here, we des...

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Bibliografiske detaljer
Main Authors: Kopanic, Jennifer L., Al-Mugotir, Mona, Zach, Sydney, Das, Srustidhar, Grosely, Rosslyn, Sorgen, Paul L.
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2013
Fag:
Pharmacology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3750199/
https://ncbi.nlm.nih.gov/pubmed/23986705
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fphar.2013.00106
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