An antisense approach to phenotype-based gene cloning in Tetrahymena
We report a pioneering approach using Tetrahymena thermophila that permits rapid identification of genes based on their null or hypomorphic phenotypes. This technique involves cell transformation with a library of plasmids that encode 26S ribosomal subunits containing short insertions. The insertion...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC37500/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11438705/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.151243498 |
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