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An antisense approach to phenotype-based gene cloning in Tetrahymena
We report a pioneering approach using Tetrahymena thermophila that permits rapid identification of genes based on their null or hypomorphic phenotypes. This technique involves cell transformation with a library of plasmids that encode 26S ribosomal subunits containing short insertions. The insertion...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC37500/ https://ncbi.nlm.nih.gov/pubmed/11438705 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.151243498 |
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