Impact of Library Preparation on Downstream Analysis and Interpretation of RNA-Seq Data: Comparison between Illumina PolyA and NuGEN Ovation Protocol

OBJECTIVES: The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses...

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Detalhes bibliográficos
Main Authors: Sun, Zhifu, Asmann, Yan W., Nair, Asha, Zhang, Yuji, Wang, Liguo, Kalari, Krishna R., Bhagwate, Aditya V., Baker, Tiffany R., Carr, Jennifer M., Kocher, Jean-Pierre A., Perez, Edith A., Thompson, E. Aubrey
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3747248/
https://ncbi.nlm.nih.gov/pubmed/23977132
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0071745
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