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Soluble Variants of Human Recombinant Glutaminyl Cyclase
Recombinant human Glutaminyl Cyclase expressed in E. coli is produced as inclusion bodies. Lack of glycosylation is the main origin of its accumulation in insoluble aggregates. Mutation of single isolated hydrophobic amino acids into negative amino acids was not able to circumvent inclusion bodies f...
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| Hlavní autoři: | , , , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Public Library of Science
2013
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3744504/ https://ncbi.nlm.nih.gov/pubmed/23977104 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0071657 |
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