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Application of Fluorescence Resonance Energy Transfer to the GroEL–GroES Chaperonin Reaction
Fluorescence resonance energy transfer (FRET) is a sensitive and flexible method for studying protein-protein interactions. Here it is applied to the GroEL-GroES chaperonin system to examine the ATP-driven dynamics that underlie protein folding by this chaperone. Relying on the known structures of G...
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2001
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| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3744193/ https://ncbi.nlm.nih.gov/pubmed/11403576 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1006/meth.2001.1188 |
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