High-Resolution Enzymatic Mapping of Genomic 5-Hydroxymethylcytosine in Mouse Embryonic Stem Cells

We describe the use of a unique DNA-modification-dependent restriction endonuclease AbaSI coupled with sequencing (Aba-seq) to map high-resolution hydroxymethylome of mouse E14 embryonic stem cells. The specificity of AbaSI enables sensitive detection of 5-hydroxymethylcytosine (5hmC) at low-occupan...

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Detalhes bibliográficos
Main Authors: Sun, Zhiyi, Terragni, Jolyon, Borgaro, Janine G., Liu, Yiwei, Yu, Ling, Guan, Shengxi, Wang, Hua, Sun, Dapeng, Cheng, Xiaodong, Zhu, Zhenyu, Pradhan, Sriharsa, Zheng, Yu
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3743234/
https://ncbi.nlm.nih.gov/pubmed/23352666
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.celrep.2013.01.001
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