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Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging()
A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photo...
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| Autors principals: | , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2012
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3742097/ https://ncbi.nlm.nih.gov/pubmed/22517076 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c2cc30373f |
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