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Shortening of alkaline DNA unwinding time does not interfere with detecting DNA damage to mouse and human spermatozoa in the comet assay
The comet assay was performed on mouse and human spermatozoa to examine the effect of alkaline DNA unwinding time. The spermatozoa were treated in vitro with the DNA-damaging agents, methyl methanesulfonate (MMS) or hydrogen peroxide (H(2)O(2)), and then embedded in agarose gel on glass slides. The...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2011
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3739381/ https://ncbi.nlm.nih.gov/pubmed/21057514 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/aja.2010.105 |
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