CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli

Prokaryotes immunize themselves against transmissible genetic elements by the integration (acquisition) in clustered regularly interspaced short palindromic repeats (CRISPR) loci of spacers homologous to invader nucleic acids, defined as protospacers. Following acquisition, mono-spacer CRISPR RNAs (...

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Hlavní autoři: Díez-Villaseñor, César, Guzmán, Noemí M., Almendros, Cristóbal, García-Martínez, Jesús, Mojica, Francisco J.M.
Médium: Artigo
Jazyk:Inglês
Vydáno: Landes Bioscience 2013
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Research Paper
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3737337/
https://ncbi.nlm.nih.gov/pubmed/23445770
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.24023
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