A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements

We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed nucleotide elements (LINE). The PCR product, which represents a pool of approximately 15 000 genomic l...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Yang, Allen S., Estécio, Marcos R. H., Doshi, Ketan, Kondo, Yutaka, Tajara, Eloiza H., Issa, Jean-Pierre J.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 2004
Schlagworte:	NAR Methods Online
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC373427/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14973332/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh032
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!