A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements

We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed nucleotide elements (LINE). The PCR product, which represents a pool of approximately 15 000 genomic l...

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Библиографические подробности

Опубликовано в::	Nucleic Acids Res
Главные авторы:	Yang, Allen S., Estécio, Marcos R. H., Doshi, Ketan, Kondo, Yutaka, Tajara, Eloiza H., Issa, Jean-Pierre J.
Формат:	Artigo
Язык:	Inglês
Опубликовано:	Oxford University Press 2004
Предметы:	NAR Methods Online
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC373427/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14973332/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gnh032
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