A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements

We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed nucleotide elements (LINE). The PCR product, which represents a pool of approximately 15 000 genomic l...

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Bibliographic Details
Main Authors: Yang, Allen S., Estécio, Marcos R. H., Doshi, Ketan, Kondo, Yutaka, Tajara, Eloiza H., Issa, Jean-Pierre J.
Format: Artigo
Language:Inglês
Published: Oxford University Press 2004
Subjects:
NAR Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC373427/
https://ncbi.nlm.nih.gov/pubmed/14973332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh032
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