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Detection of L-Lactate In Polyethylene Glycol Solutions Confirms Identity of Active Site Ligand in Proline Dehydrogenase Structure
Polyethylene glycol (PEG) is often used in protein crystallography as a low ionic strength precipitant for crystallization and a cryoprotectant for low temperature data collection. Prompted by the discovery of an apparent L-lactate molecule bound in the active site of the E. coli PutA proline dehydr...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2004
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3733669/ https://ncbi.nlm.nih.gov/pubmed/15103160 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0907444904003786 |
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