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Cryoprotection mechanisms of polyethylene glycols on lactate dehydrogenase during freeze-thawing

The purpose of this study was to explore the cryoprotection mechanisms of high molecular weight polyethylene glycols (PEGs) (eg, PEG 4000 and PEG 8000) on lactate dehydrogenase (LDH). Ultraviolet activity assays, circular dichroism (CD) spectroscopy, gel filtration, sodium dodecyl sulfate polyacryla...

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Main Authors: Mi, Yanli, Wood, George, Thoma, Laura
Format: Artigo
Jezik:Inglês
Izdano: Springer US 2004
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2751247/
https://ncbi.nlm.nih.gov/pubmed/15760107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1208/aapsj060322
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