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Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor
We report for the first time the in vitro characterization of a reverse tetracycline repressor (revTetR). The dimeric wild-type repressor (TetR) binds to tet operator tetO in the absence of the inducer anhydrotetracycline (atc) to confer tight repression. We have isolated the revTetR G96E L205S muta...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC373327/ https://ncbi.nlm.nih.gov/pubmed/14764926 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkh200 |
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