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Tetracycline-Dependent Conditional Gene Knockout in Bacillus subtilis

Reversible tetracycline-dependent gene regulation allows induction of expression with the tetracycline repressor (TetR) or gene silencing with the newly developed reverse mutant revTetR. We report here the implementation of both approaches with full regulatory range in gram-positive bacteria as exem...

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Detalhes bibliográficos
Main Authors: Kamionka, Annette, Bertram, Ralph, Hillen, Wolfgang
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC546824/
https://ncbi.nlm.nih.gov/pubmed/15691923
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.2.728-733.2005
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