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Tetracycline-Dependent Conditional Gene Knockout in Bacillus subtilis
Reversible tetracycline-dependent gene regulation allows induction of expression with the tetracycline repressor (TetR) or gene silencing with the newly developed reverse mutant revTetR. We report here the implementation of both approaches with full regulatory range in gram-positive bacteria as exem...
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Main Authors: | , , |
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Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
American Society for Microbiology
2005
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Fag: | |
Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC546824/ https://ncbi.nlm.nih.gov/pubmed/15691923 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.2.728-733.2005 |
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