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Tetracycline-Dependent Conditional Gene Knockout in Bacillus subtilis

Reversible tetracycline-dependent gene regulation allows induction of expression with the tetracycline repressor (TetR) or gene silencing with the newly developed reverse mutant revTetR. We report here the implementation of both approaches with full regulatory range in gram-positive bacteria as exem...

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Bibliografiske detaljer
Main Authors: Kamionka, Annette, Bertram, Ralph, Hillen, Wolfgang
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2005
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC546824/
https://ncbi.nlm.nih.gov/pubmed/15691923
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.2.728-733.2005
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