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Superior 5′ homogeneity of RNA from ATP-initiated transcription under the T7 φ2.5 promoter
Transcription from the commonly used GTP- initiating T7 class III promoter φ6.5 frequently produces heterogeneous RNA at both 3′ and 5′ ends. We demonstrate here that RNA transcripts from the T7 class II promoter φ2.5 have superior 5′ homogeneity over those from the φ6.5 promoter, with comparable to...
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| Autores principales: | , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2004
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC373309/ https://ncbi.nlm.nih.gov/pubmed/14744982 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gnh007 |
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