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Dorsomorphin Promotes Survival and Germline Competence of Zebrafish Spermatogonial Stem Cells in Culture

Zebrafish spermatogonial cell cultures were established from Tg(piwil1:neo);Tg(piwil1:DsRed) transgenic fish using a zebrafish ovarian feeder cell line (OFC3) that was engineered to express zebrafish Lif, Fgf2 and Gdnf. Primary cultures, initiated from testes, were treated with G418 to eliminate the...

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Detalles Bibliográficos
Main Authors: Wong, Ten-Tsao, Collodi, Paul
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2013
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3731312/
https://ncbi.nlm.nih.gov/pubmed/23936500
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0071332
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