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Transposon mutagenesis of Bacteroides fragilis using a Mariner transposon vector

The mariner transposon vector pYV07 was tested for use in the mutagenesis of Bacteroides fragilis 638R. The transposon vector efficiently generated mutants in B. fragilis 638R. The transposon disrupted genes were scattered throughout the genome of B. fragilis 638R. This method serves as a powerful t...

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Main Authors: Veeranagouda, Yaligara, Husain, Fasahath, Wexler, Hannah M.
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3729920/
https://ncbi.nlm.nih.gov/pubmed/23664906
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.anaerobe.2013.04.012
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