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Transposon mutagenesis of the anaerobic commensal, Bacteroides fragilis, using the EZ∷TN5 transposome
Genetic analysis of Bacteroides fragilis (BF) is hindered due to the lack of efficient transposon mutagenesis methods. Here we describe a simple method for transposon mutagenesis using EZ∷TN5, a commercially available system that we optimized for use in BF638R. The modified EZ∷TN5 transposon contain...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2012
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3500642/ https://ncbi.nlm.nih.gov/pubmed/22639975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1574-6968.2012.02602.x |
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