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Derivation of human embryonic stem cell lines from single blastomeres of low-quality embryos by direct plating

PURPOSE: To explore a simple method of establishing pluripotent human embryonic stem cell (hESC) lines from single blastomeres of low-quality (LQ) embryos. METHODS: Blastomeres were isolated from normally fertilized, day-3 pre-implantation LQ embryos by dissolving of the zona pellucida and were then...

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Detalhes bibliográficos
Main Authors: Yang, Gang, Mai, Qingyun, Li, Tao, Zhou, Canquan
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3725230/
https://ncbi.nlm.nih.gov/pubmed/23835723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10815-013-0042-x
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