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Quantitative Analysis of Histone Demethylase Probes Using Fluorescence Polarization
We previously reported methylstat as a selective inhibitor of jumonji C domain-containing histone de-methylases (JHDMs). Herein, we describe the synthesis of a fluorescent analogue of methylstat and its application as a tracer in fluorescence polarization assays. Using this format, we have evaluated...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3724763/ https://ncbi.nlm.nih.gov/pubmed/23725560 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jm3018628 |
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