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Cloning-independent plasmid construction for genetic studies in streptococci
Shuttle plasmids are among the few routinely utilized tools in the Streptococcus mutans genetic system that still require the use of classical cloning methodologies and intermediate hosts for genetic manipulation. Accordingly, it typically requires considerably less time and effort to introduce muta...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3720822/ https://ncbi.nlm.nih.gov/pubmed/23673081 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2013.05.005 |
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