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Cloning-independent plasmid construction for genetic studies in streptococci

Shuttle plasmids are among the few routinely utilized tools in the Streptococcus mutans genetic system that still require the use of classical cloning methodologies and intermediate hosts for genetic manipulation. Accordingly, it typically requires considerably less time and effort to introduce muta...

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Autors principals: Xie, Zhoujie, Qi, Fengxia, Merritt, Justin
Format: Artigo
Idioma:Inglês
Publicat: 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3720822/
https://ncbi.nlm.nih.gov/pubmed/23673081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2013.05.005
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