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Evolution of a designed retro-aldolase leads to complete active site remodeling

Evolutionary advances are often fueled by unanticipated innovation. Directed evolution of a computationally designed enzyme suggests that dramatic molecular changes can also drive the optimization of primitive protein active sites. The specific activity of an artificial retro-aldolase was boosted &g...

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Bibliografske podrobnosti
Main Authors: Giger, Lars, Caner, Sami, Obexer, Richard, Kast, Peter, Baker, David, Ban, Nenad, Hilvert, Donald
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3720730/
https://ncbi.nlm.nih.gov/pubmed/23748672
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.1276
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