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Fusion of a Flavin-Based Fluorescent Protein to Hydroxynitrile Lyase from Arabidopsis thaliana Improves Enzyme Stability

Hydroxynitrile lyase from Arabidopsis thaliana (AtHNL) was fused to different fluorescent reporter proteins. Whereas all fusion constructs retained enzymatic activity and fluorescence in vivo and in vitro, significant differences in activity and pH stability were observed. In particular, flavin-base...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Scholz, Kathrin Emmi, Kopka, Benita, Wirtz, Astrid, Pohl, Martina, Jaeger, Karl-Erich, Krauss, Ulrich
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 2013
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3719508/
https://ncbi.nlm.nih.gov/pubmed/23728815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00795-13
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