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Fusion of a Flavin-Based Fluorescent Protein to Hydroxynitrile Lyase from Arabidopsis thaliana Improves Enzyme Stability

Hydroxynitrile lyase from Arabidopsis thaliana (AtHNL) was fused to different fluorescent reporter proteins. Whereas all fusion constructs retained enzymatic activity and fluorescence in vivo and in vitro, significant differences in activity and pH stability were observed. In particular, flavin-base...

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מידע ביבליוגרפי
Main Authors: Scholz, Kathrin Emmi, Kopka, Benita, Wirtz, Astrid, Pohl, Martina, Jaeger, Karl-Erich, Krauss, Ulrich
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology 2013
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3719508/
https://ncbi.nlm.nih.gov/pubmed/23728815
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00795-13
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