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A Redox Trap to Augment the Intein Toolbox

The unregulated activity of inteins during expression and consequent side reactions during work-up limits their widespread use in biotechnology and chemical biology. Therefore, we exploited a mechanism-based approach to regulate intein autocatalysis for biotechnological application. The system, insp...

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Detalhes bibliográficos
Main Authors: Callahan, Brian P., Stanger, Matthew, Belfort, Marlene
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3718494/
https://ncbi.nlm.nih.gov/pubmed/23280506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.24821
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