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Structure of catalytically competent intein caught in a redox trap with functional and evolutionary implications

Inteins self-splice from precursor polypeptides to reconstitute functional proteins. Here we describe inteins as redox-responsive switches in bacteria. Regulation was achieved by engineering a disulfide bond between the intein’s catalytic cysteine and the flanking polypeptide. This interaction was v...

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Detalhes bibliográficos
Main Authors: Callahan, Brian P., Topilina, Natalya I., Stanger, Matthew J., Roey, Patrick Van, Belfort, Marlene
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3087850/
https://ncbi.nlm.nih.gov/pubmed/21460844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nsmb.2041
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