Single-molecule and transient kinetics investigation of the interaction of dihydrofolate reductase with NADPH and dihydrofolate

The interaction of dihydrofolate (H(2)F) and NADPH with a fluorescent derivative of H(2)F reductase (DHFR) was studied by using transient and single-molecule techniques. The fluorescent moiety Alexa 488 was attached to the structural loop that closes over the substrates after they are bound. Fluores...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Zhang, Zhiquan, Rajagopalan, P. T. Ravi, Selzer, Tzvia, Benkovic, Stephen J., Hammes, Gordon G.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 2004
Soggetti:	Biological Sciences
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC371724/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14978269/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0400091101
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