RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction

BACKGROUND: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement. However, the RNA-seq sequence data can be biased during library constructions...

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Bibliografiska uppgifter
Huvudupphovsmän: Lee, Changhoon, Harris, R Adron, Wall, Jason K, Mayfield, R Dayne, Wilke, Claus O
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2013
Ämnen:
Research
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3710281/
https://ncbi.nlm.nih.gov/pubmed/23826734
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1745-6150-8-16
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