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RNaseIII and T4 Polynucleotide Kinase sequence biases and solutions during RNA-seq library construction
BACKGROUND: RNA-seq is a next generation sequencing method with a wide range of applications including single nucleotide polymorphism (SNP) detection, splice junction identification, and gene expression level measurement. However, the RNA-seq sequence data can be biased during library constructions...
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Main Authors: | , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
BioMed Central
2013
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3710281/ https://ncbi.nlm.nih.gov/pubmed/23826734 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1745-6150-8-16 |
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