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Comparison of Strong Cation Exchange and SDS/PAGE Fractionation for Analysis of Multi-Protein Complexes

Affinity purification of protein complexes followed by identification using liquid chromatography/mass spectrometry (LC-MS/MS) is a robust method to study the fundamental process of protein interaction. While affinity isolation reduces the complexity of the sample, fractionation prior to LC-MS/MS an...

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Detalhes bibliográficos
Main Authors: Das, Sudipto, Bosley, Allen D., Ye, Xiaoying, Chan, King C., Chu, Isabel, Green, Jeffery E., Issaq, Haleem J., Veenstra, Timothy D., Andresson, Thorkell
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3707127/
https://ncbi.nlm.nih.gov/pubmed/20968308
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr100843x
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