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Increasing Proteome Coverage with Offline RP HPLC Coupled to Online RP NanoLC-MS
Fractionation prior to mass spectrometry is an indispensable step in proteomics. In this paper we report the success of performing offline reversed phase high pressure liquid chromatography (HPLC) fractionation on a C18 2.0 mm × 150 mm column at the peptide level with microliter per minute flow rate...
Tallennettuna:
| Päätekijät: | , , , |
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| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
2011
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3703961/ https://ncbi.nlm.nih.gov/pubmed/21342794 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2011.01.032 |
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