A detergent-free strategy for the reconstitution of active enzyme complexes from native biological membranes into nanoscale discs

BACKGROUND: The reconstitution of membrane proteins and complexes into nanoscale lipid bilayer structures has contributed significantly to biochemical and biophysical analyses. Current methods for performing such reconstitutions entail an initial detergent-mediated step to solubilize and isolate mem...

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Détails bibliographiques
Auteurs principaux: Long, Ashley R, O’Brien, Catherine C, Malhotra, Ketan, Schwall, Christine T, Albert, Arlene D, Watts, Anthony, Alder, Nathan N
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2013
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3702409/
https://ncbi.nlm.nih.gov/pubmed/23663692
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-41
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