Coaligned Dual-Channel STED Nanoscopy and Molecular Diffusion Analysis at 20 nm Resolution

We report on a fiber laser-based stimulated emission-depletion microscope providing down to ∼20 nm resolution in raw data images as well as 15–19 nm diameter probing areas in fluorescence correlation spectroscopy. Stimulated emission depletion pulses of nanosecond duration and 775 nm wavelength are...

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Detalhes bibliográficos
Main Authors: Göttfert, Fabian, Wurm, Christian A., Mueller, Veronika, Berning, Sebastian, Cordes, Volker C., Honigmann, Alf, Hell, Stefan W.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2013
Assuntos:
Biophysical Letter
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3699760/
https://ncbi.nlm.nih.gov/pubmed/23823248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2013.05.029
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