Two-photon STED nanoscopy realizing 100-nm spatial resolution utilizing high-peak-power sub-nanosecond 655-nm pulses

We developed two-photon excitation stimulated emission depletion (STED) nanoscopy using high-peak-power sub-nanosecond 655-nm pulses. The STED pulse exhibited ideal optical properties and sufficient pulse energy to realize a 70-nm spatial resolution in the compact setup with electrically controllabl...

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Veröffentlicht in:Biomed Opt Express
Hauptverfasser: Ishii, Hirokazu, Otomo, Kohei, Hung, Jui-Hung, Tsutsumi, Motosuke, Yokoyama, Hiroyuki, Nemoto, Tomomi
Format: Artigo
Sprache:Inglês
Veröffentlicht: Optical Society of America 2019
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6706030/
https://ncbi.nlm.nih.gov/pubmed/31467771
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.10.003104
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