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Metabolism of Peptide Reporters in Cell Lysates and Single Cells
The stability of an Abl kinase substrate peptide in a cytosolic lysate and in single cells was characterized. In the cytosolic lysate, the starting peptide was metabolized at an average initial rate of 1.7 ± 0.3 zmol pg(−;1) s(−;1) with a t(1/2) of 1.3 min. Five different fragments formed over time;...
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Main Authors: | , , , |
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Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
2012
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Fag: | |
Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3697743/ https://ncbi.nlm.nih.gov/pubmed/22314840 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c2an16162a |
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