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Massive functional mapping of a 5′-UTR by saturation mutagenesis, phenotypic sorting and deep sequencing

We present here a method that enables functional screening of large number of mutations in a single experiment through the combination of random mutagenesis, phenotypic cell sorting and high-throughput sequencing. As a test case, we studied post-transcriptional gene regulation of the bacterial csgD...

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Detalhes bibliográficos
Main Authors: Holmqvist, Erik, Reimegård, Johan, Wagner, E. Gerhart H.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3695526/
https://ncbi.nlm.nih.gov/pubmed/23609548
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt267
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