Sequencing viral genomes from a single isolated plaque

BACKGROUND: Whole genome sequencing of viruses and bacteriophages is often hindered because of the need for large quantities of genomic material. A method is described that combines single plaque sequencing with an optimization of Sequence Independent Single Primer Amplification (SISPA). This method...

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Autori principali: DePew, Jessica, Zhou, Bin, McCorrison, Jamison M, Wentworth, David E, Purushe, Janaki, Koroleva, Galina, Fouts, Derrick E
Natura: Artigo
Lingua:Inglês
Pubblicazione: BioMed Central 2013
Soggetti:
Methodology
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3693891/
https://ncbi.nlm.nih.gov/pubmed/23742765
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-10-181
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