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Purification of vacuoles from Neurospora crassa.

The Neurospora crassa vacuole, defined by its content of basic amino acids, polyphosphate, protease, phosphatases, and alpha-mannosidase, was purified to near homogeneity. The procedure depends upon homogenization of snail gut enzyme-digested cells in a buffer osmotically stabilized with 1 M sorbito...

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Detalhes bibliográficos
Main Authors: Vaughn, L E, Davis, R H
Formato: Artigo
Idioma:Inglês
Publicado em: 1981
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC369363/
https://ncbi.nlm.nih.gov/pubmed/9279392
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