Strategies for folding of affinity tagged proteins using GroEL and osmolytes

Obtaining a proper fold of affinity tagged chimera proteins can be difficult. Frequently, the protein of interest aggregates after the chimeric affinity tag is cleaved off, even when the entire chimeric construct is initially soluble. If the attached protein is incorrectly folded, chaperone proteins...

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Detalhes bibliográficos
Main Authors: Katayama, Hiroo, McGill, Mitchell, Kearns, Andrew, Brzozowski, Marek, Degner, Nicholas, Harnett, Bliss, Kornilayev, Boris, Matkovic-Calogovic, Dubravka, Holyoak, Todd, Calvet, James P., Gogol, Edward P., Seed, John, Fisher, Mark T.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3693453/
https://ncbi.nlm.nih.gov/pubmed/19082872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10969-008-9053-8
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