Transfer of herpes simplex virus thymidine kinase synthesized in bacteria by a high-expression plasmid to tissue culture cells by protoplast fusion.
The introduction of a protein into living tissue culture cells may permit the in vivo study of functions of the protein. We have previously described a high-efficiency-expression plasmid, pHETK2, containing the herpes simplex virus type 1 thymidine kinase (TK) gene which, upon temperature induction,...
Guardat en:
| Publicat a: | Mol Cell Biol |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Taylor & Francis
1984
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368961/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6092926/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.4.8.1644-1646.1984 |
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