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Short-term, high-efficiency expression of transfected DNA.
We have achieved high-efficiency uptake and expression of foreign DNA in mouse Ltk- cells by modifying the DEAE-dextran-mediated transfection method of McCutchan and Pagano (J. Natl. Cancer Inst. 42:351-357, 1968) to include an initial incubation at elevated pH followed by a shock treatment with dim...
Gespeichert in:
| Veröffentlicht in: | Mol Cell Biol |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Taylor & Francis
1984
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368960/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6092925/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.4.8.1641 |
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