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BK virus-plasmid expression vector that persists episomally in human cells and shuttles into Escherichia coli.

We describe a novel expression vector, pBK TK-1, that persists episomally in human cells that can be shuttled into bacteria. This vector includes sequences from BK virus (BKV), the thymidine kinase (TK) gene of herpes simplex virus type 1, and plasmid pML-1. TK+-transformed HeLa and 143 B cells cont...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Mol Cell Biol
Hauptverfasser: Milanesi, G, Barbanti-Brodano, G, Negrini, M, Lee, D, Corallini, A, Caputo, A, Grossi, M P, Ricciardi, R P
Format: Artigo
Sprache:Inglês
Veröffentlicht: Taylor & Francis 1984
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368947/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6092918/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.4.8.1551-1560.1984
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