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BK virus-plasmid expression vector that persists episomally in human cells and shuttles into Escherichia coli.

We describe a novel expression vector, pBK TK-1, that persists episomally in human cells that can be shuttled into bacteria. This vector includes sequences from BK virus (BKV), the thymidine kinase (TK) gene of herpes simplex virus type 1, and plasmid pML-1. TK+-transformed HeLa and 143 B cells cont...

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Detalles Bibliográficos
Publicado en:Mol Cell Biol
Main Authors: Milanesi, G, Barbanti-Brodano, G, Negrini, M, Lee, D, Corallini, A, Caputo, A, Grossi, M P, Ricciardi, R P
Formato: Artigo
Idioma:Inglês
Publicado: Taylor & Francis 1984
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368947/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6092918/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.4.8.1551
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