BK virus-plasmid expression vector that persists episomally in human cells and shuttles into Escherichia coli.

We describe a novel expression vector, pBK TK-1, that persists episomally in human cells that can be shuttled into bacteria. This vector includes sequences from BK virus (BKV), the thymidine kinase (TK) gene of herpes simplex virus type 1, and plasmid pML-1. TK+-transformed HeLa and 143 B cells cont...

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Main Authors: Milanesi, G, Barbanti-Brodano, G, Negrini, M, Lee, D, Corallini, A, Caputo, A, Grossi, M P, Ricciardi, R P
格式: Artigo
語言:Inglês
出版: 1984
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC368947/
https://ncbi.nlm.nih.gov/pubmed/6092918
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