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Expression, Purification and Characterization of the Proline Dehydrogenase Domain of PutA from Pseudomonas putida POS-F84

The objective of the present work was to express a truncated form of Pseudomonas putida PutA that shows proline dehydrogenase (ProDH) activity. The putA gene encoding ProDH enzyme was cloned into pET23a vector and expressed in Escherichia coli strain BL-21 (DE3) plysS. The recombinant P. putida enzy...

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Detalhes bibliográficos
Main Authors: Omidinia, Eskandar, Mahdizadehdehosta, Rahman, Mohammadi, Hamid Shahbaz
Formato: Artigo
Idioma:Inglês
Publicado em: Springer-Verlag 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3689398/
https://ncbi.nlm.nih.gov/pubmed/24426126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12088-013-0375-2
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