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Rescue of functional replication origins from embedded configurations in a plasmid carrying the adenovirus genome.

A variant of the adenovirus type 5 genome which lacks EcoRI sites has been cloned in a bacterial plasmid after the addition of EcoRI oligonucleotide linkers to its ends. Closed circular forms of the recombinant viral genome were not infectious upon their introduction into permissive eucaryotic cells...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hanahan, D, Gluzman, Y
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1984
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC368696/
https://ncbi.nlm.nih.gov/pubmed/6700592
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