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Recombinant plasmids carrying promoters, genes and the origin of DNA replication of the early region of bacteriophage T7.
Two full-length contiguous HpaI fragments of the 0 to 18.2% region of T7 H DNA (HpF-H and HpG) were inserted into plasmids pHV14 or pC194 using oligo(dG . dC) connectors or synthetic HindIII adaptors. Amplification of the two early T7 fragments was achieved by transforming lysostaphin-treated S. aur...
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| Main Authors: | , , , |
|---|---|
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1980
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC323992/ https://ncbi.nlm.nih.gov/pubmed/7433121 |
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