Transformation of Neurospora crassa with the cloned am (glutamate dehydrogenase) gene.
We used DNA containing the am gene of Neurospora crassa, cloned in the lambda replacement vector lambdaL-47 (this clone is designated lambdaC-10), and plasmid vector subclones of this DNA to transform am deletion and point mutant strains. By means of subcloning, all sequences required for transforma...
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| Published in: | Mol Cell Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Taylor & Francis
1984
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368665/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6230518/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.4.1.117-122.1984 |
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