Carbohydrate-Binding Module–Cyclodextrin Glycosyltransferase Fusion Enables Efficient Synthesis of 2-O-d-Glucopyranosyl-l-Ascorbic Acid with Soluble Starch as the Glycosyl Donor

In this study, we achieved the efficient synthesis of 2-O-d-glucopyranosyl-l-ascorbic acid (AA-2G) from soluble starch by fusing a carbohydrate-binding module (CBM) from Alkalimonas amylolytica α-amylase (CBM(Amy)) to cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans. One fusion...

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Bibliographische Detailangaben
Hauptverfasser: Han, Ruizhi, Li, Jianghua, Shin, Hyun-Dong, Chen, Rachel R., Du, Guocheng, Liu, Long, Chen, Jian
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2013
Schlagworte:
Enzymology and Protein Engineering
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3685265/
https://ncbi.nlm.nih.gov/pubmed/23503312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00363-13
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