Iterative Saturation Mutagenesis of −6 Subsite Residues in Cyclodextrin Glycosyltransferase from Paenibacillus macerans To Improve Maltodextrin Specificity for 2-O-d-Glucopyranosyl-l-Ascorbic Acid Synthesis

2-O-d-Glucopyranosyl-l-ascorbic acid (AA-2G), a stable l-ascorbic acid derivative, is usually synthesized by cyclodextrin glycosyltransferase (CGTase), which contains nine substrate-binding subsites (from +2 to −7). In this study, iterative saturation mutagenesis (ISM) was performed on the −6 subsit...

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Detalhes bibliográficos
Main Authors: Han, Ruizhi, Liu, Long, Shin, Hyun-dong, Chen, Rachel R., Li, Jianghua, Du, Guocheng, Chen, Jian
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2013
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3837798/
https://ncbi.nlm.nih.gov/pubmed/24077706
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02918-13
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